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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
12/09/2018 |
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Data da última atualização: |
12/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HAACH, V.; GAVA, D.; COLDEBELLA, A.; SCHAEFER, R. |
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Afiliação: |
VANESSA HAACH, CNPq; DANIELLE GAVA, CNPSA; ARLEI COLDEBELLA, CNPSA; REJANE SCHAEFER, CNPSA. |
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Título: |
Influência das condições de armazenamento do vírus influenza na detecção por RT-qPCR e isolamento viral. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Ciência Animal Brasileira, Goiânia, v.19, p. 1-9, e-4689, 2018. |
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Idioma: |
Português |
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Conteúdo: |
Resumo: A infecção de suínos pelo vírus influenza causa perdas significativas na suinocultura e a doença tem implicações consideráveis para a saúde pública. Dessa forma, a rápida detecção viral em amostras biológicas de suínos é importante para a vigilância da influenza. Para o diagnóstico, as condições de manutenção das amostras biológicas (modo de acondicionamento, temperatura e período de acondicionamento), desde a colheita das amostras de suínos até o envio ao laboratório, podem interferir negativamente na detecção viral. Neste estudo foi analisada a viabilidade de uma amostra do vírus influenza A H1N1/2009 isolada de suínos, mantida em diferentes modos de acondicionamento (meio comercial UTM, meio in house VTM e sem meio de manutenção) e diferentes temperaturas (4, 23 e 37 °C) por um período de até 120 horas. As amostras foram avaliadas por RT-qPCR e isolamento em ovos embrionados. Foram observados efeitos significativos (p<0,05) para o modo e período de acondicionamento e da interação entre esses dois fatores com a carga viral. Dessa forma, as amostras biológicas enviadas para diagnóstico de influenza devem ser armazenadas, preferencialmente, em meio de manutenção viral a 4 °C e o tempo decorrido entre a colheita da amostra e a chegada ao laboratório deve ser de, no máximo, três dias. Abstract: Influenza virus infection in pigs causes significant losses for the swine industry and concerns for the public health. Therefore, rapid virus detection is important for influenza surveillance in pigs. Storage conditions (such as medium, temperature, and time) of the biological samples are very important for the diagnosis because they can negatively interfere with the viral detection. In this study, influenza virus viability was evaluated in different storage media (UTM commercial medium, ?in house? VTM medium, and without storage medium), temperature (4, 23 and 37 °C), and storage time (up to 120 hours). Samples were evaluated by RT-qPCR and isolation in embryonated chicken eggs. Significant effects (p<0.05) were observed for the media and time besides the interaction between the two factors with the viral load. In conclusion, biological samples of pigs sent for influenza diagnosis should be stored, preferably in viral maintenance medium at 4 °C, and the time estimated between the sample collection until the arrival in the laboratory should be less than three days. MenosResumo: A infecção de suínos pelo vírus influenza causa perdas significativas na suinocultura e a doença tem implicações consideráveis para a saúde pública. Dessa forma, a rápida detecção viral em amostras biológicas de suínos é importante para a vigilância da influenza. Para o diagnóstico, as condições de manutenção das amostras biológicas (modo de acondicionamento, temperatura e período de acondicionamento), desde a colheita das amostras de suínos até o envio ao laboratório, podem interferir negativamente na detecção viral. Neste estudo foi analisada a viabilidade de uma amostra do vírus influenza A H1N1/2009 isolada de suínos, mantida em diferentes modos de acondicionamento (meio comercial UTM, meio in house VTM e sem meio de manutenção) e diferentes temperaturas (4, 23 e 37 °C) por um período de até 120 horas. As amostras foram avaliadas por RT-qPCR e isolamento em ovos embrionados. Foram observados efeitos significativos (p<0,05) para o modo e período de acondicionamento e da interação entre esses dois fatores com a carga viral. Dessa forma, as amostras biológicas enviadas para diagnóstico de influenza devem ser armazenadas, preferencialmente, em meio de manutenção viral a 4 °C e o tempo decorrido entre a colheita da amostra e a chegada ao laboratório deve ser de, no máximo, três dias. Abstract: Influenza virus infection in pigs causes significant losses for the swine industry and concerns for the public health. Therefore, rapid virus detection is important for influenz... Mostrar Tudo |
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Palavras-Chave: |
H1N1 virus. |
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Thesagro: |
Diagnostico; Suíno; Virologia. |
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Thesaurus Nal: |
Influenza A virus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182810/1/final8902.pdf
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Marc: |
LEADER 03097naa a2200217 a 4500
001 2095527
005 2018-09-12
008 2018 bl uuuu u00u1 u #d
100 1 $aHAACH, V.
245 $aInfluência das condições de armazenamento do vírus influenza na detecção por RT-qPCR e isolamento viral.$h[electronic resource]
260 $c2018
520 $aResumo: A infecção de suínos pelo vírus influenza causa perdas significativas na suinocultura e a doença tem implicações consideráveis para a saúde pública. Dessa forma, a rápida detecção viral em amostras biológicas de suínos é importante para a vigilância da influenza. Para o diagnóstico, as condições de manutenção das amostras biológicas (modo de acondicionamento, temperatura e período de acondicionamento), desde a colheita das amostras de suínos até o envio ao laboratório, podem interferir negativamente na detecção viral. Neste estudo foi analisada a viabilidade de uma amostra do vírus influenza A H1N1/2009 isolada de suínos, mantida em diferentes modos de acondicionamento (meio comercial UTM, meio in house VTM e sem meio de manutenção) e diferentes temperaturas (4, 23 e 37 °C) por um período de até 120 horas. As amostras foram avaliadas por RT-qPCR e isolamento em ovos embrionados. Foram observados efeitos significativos (p<0,05) para o modo e período de acondicionamento e da interação entre esses dois fatores com a carga viral. Dessa forma, as amostras biológicas enviadas para diagnóstico de influenza devem ser armazenadas, preferencialmente, em meio de manutenção viral a 4 °C e o tempo decorrido entre a colheita da amostra e a chegada ao laboratório deve ser de, no máximo, três dias. Abstract: Influenza virus infection in pigs causes significant losses for the swine industry and concerns for the public health. Therefore, rapid virus detection is important for influenza surveillance in pigs. Storage conditions (such as medium, temperature, and time) of the biological samples are very important for the diagnosis because they can negatively interfere with the viral detection. In this study, influenza virus viability was evaluated in different storage media (UTM commercial medium, ?in house? VTM medium, and without storage medium), temperature (4, 23 and 37 °C), and storage time (up to 120 hours). Samples were evaluated by RT-qPCR and isolation in embryonated chicken eggs. Significant effects (p<0.05) were observed for the media and time besides the interaction between the two factors with the viral load. In conclusion, biological samples of pigs sent for influenza diagnosis should be stored, preferably in viral maintenance medium at 4 °C, and the time estimated between the sample collection until the arrival in the laboratory should be less than three days.
650 $aInfluenza A virus
650 $aDiagnostico
650 $aSuíno
650 $aVirologia
653 $aH1N1 virus
700 1 $aGAVA, D.
700 1 $aCOLDEBELLA, A.
700 1 $aSCHAEFER, R.
773 $tCiência Animal Brasileira, Goiânia$gv.19, p. 1-9, e-4689, 2018.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
09/01/2019 |
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Data da última atualização: |
30/01/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LUCON, I. M.; COSCIONE, A. R.; ANDRADE, C. A. de. |
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Afiliação: |
Isabella Menuzzo Lucon, IAC; Aline Renée Coscione, IAC; CRISTIANO ALBERTO DE ANDRADE, CNPMA. |
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Título: |
Eucalyptus biochar soil fertility and corn yield on a long term field experiment. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: WORLD CONGRESS OF SOIL SCIENCE, 21., 2018, Rio de Janeiro. Soil science: beyond food and fuel: abstracts. Viçosa, MG: SBCS, 2018. Trabalho 1545. |
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Idioma: |
Português |
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Conteúdo: |
Eucalyptus wood char is a renewable energy source produced by partial wood carbonization on mud ovens close to the plantation areas, and is an important alternative to mineral char mainly in the metallurgy industry. This process generates a black solid by-product, the wood biochar (WB), collect after many char batches production, form the bottom of the ovens. Most studies with biochar occur under controlled environments. The field experimentation makes it possible to obtain more concrete data about its effects long term either in soil and agricultural production, over time. This research is being developed at Agronomic Institute (IAC), Campinas/SP, in a clayey oxisoil with six doses of WB: Control, without WB; 10, 20, 30, 40 and 50 Mg ha-1 WB, which were applied on April 2016. The pHH2O (1:10) of WB is 7.5. The WB doses used in the experimentation corresponds up to 25 Mg ha-1 of C; 1.4 Mg ha-1 Ca; 470 kg ha-1 of N; 75 kg ha-1 of P; 320 kg ha-1 of K and 65 kg ha-1 of Mg, at the 0-20 cm layer. NPK fertilization occurred in the corn planting, in line, below the seeds, on December 2017. The soil was collected in June 2017, after the harvest. Macronutrients, micronutrients and heavy metals contents were determinate in the diagnostic leaves of the plants as well as the shoots dry matter and corn yield. Soil organic matter (SOM), macro and micronutrients contents, pH and CEC were determinate in the soil. Nitrogen contents in the plants ranged from 28 to 30 g kg-1; while P ranged from 1.8 to 2 g kg-1 and K from 19 to 20 g kg-1. However no significative effects were identified for such elements in the diagnostic leaves. Furthermore, neither the corm yield nor the dry matter shoots were affected by the addition of WB to the soil. There was a small increase in the soil pH and CEC for the higher doses of WB, but also it were not significative. SOM did not increase with the WB applied doses, indicating that the increase of WB did not provide acceleration in SOM decomposition neither was mineralized. The results indicate that, after 14 months of soil application, WB aging in soil was not significant. In addition, the WB showed low neutralization power in soil, which is often with biochar application in tropical soils. MenosEucalyptus wood char is a renewable energy source produced by partial wood carbonization on mud ovens close to the plantation areas, and is an important alternative to mineral char mainly in the metallurgy industry. This process generates a black solid by-product, the wood biochar (WB), collect after many char batches production, form the bottom of the ovens. Most studies with biochar occur under controlled environments. The field experimentation makes it possible to obtain more concrete data about its effects long term either in soil and agricultural production, over time. This research is being developed at Agronomic Institute (IAC), Campinas/SP, in a clayey oxisoil with six doses of WB: Control, without WB; 10, 20, 30, 40 and 50 Mg ha-1 WB, which were applied on April 2016. The pHH2O (1:10) of WB is 7.5. The WB doses used in the experimentation corresponds up to 25 Mg ha-1 of C; 1.4 Mg ha-1 Ca; 470 kg ha-1 of N; 75 kg ha-1 of P; 320 kg ha-1 of K and 65 kg ha-1 of Mg, at the 0-20 cm layer. NPK fertilization occurred in the corn planting, in line, below the seeds, on December 2017. The soil was collected in June 2017, after the harvest. Macronutrients, micronutrients and heavy metals contents were determinate in the diagnostic leaves of the plants as well as the shoots dry matter and corn yield. Soil organic matter (SOM), macro and micronutrients contents, pH and CEC were determinate in the soil. Nitrogen contents in the plants ranged from 28 to 30 g kg-1; while P ranged fr... Mostrar Tudo |
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Palavras-Chave: |
By-product. |
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Thesagro: |
Eucalipto; Fertilidade do Solo; Milho; Oxisol. |
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Thesaurus NAL: |
soil organic matter. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/190313/1/RA-AndradeCA-21WCSS-2018-Trabalho-1545.pdf
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Marc: |
LEADER 02940nam a2200205 a 4500
001 2103363
005 2019-01-30
008 2018 bl uuuu u00u1 u #d
100 1 $aLUCON, I. M.
245 $aEucalyptus biochar soil fertility and corn yield on a long term field experiment.$h[electronic resource]
260 $aIn: WORLD CONGRESS OF SOIL SCIENCE, 21., 2018, Rio de Janeiro. Soil science: beyond food and fuel: abstracts. Viçosa, MG: SBCS, 2018. Trabalho 1545.$c1545
520 $aEucalyptus wood char is a renewable energy source produced by partial wood carbonization on mud ovens close to the plantation areas, and is an important alternative to mineral char mainly in the metallurgy industry. This process generates a black solid by-product, the wood biochar (WB), collect after many char batches production, form the bottom of the ovens. Most studies with biochar occur under controlled environments. The field experimentation makes it possible to obtain more concrete data about its effects long term either in soil and agricultural production, over time. This research is being developed at Agronomic Institute (IAC), Campinas/SP, in a clayey oxisoil with six doses of WB: Control, without WB; 10, 20, 30, 40 and 50 Mg ha-1 WB, which were applied on April 2016. The pHH2O (1:10) of WB is 7.5. The WB doses used in the experimentation corresponds up to 25 Mg ha-1 of C; 1.4 Mg ha-1 Ca; 470 kg ha-1 of N; 75 kg ha-1 of P; 320 kg ha-1 of K and 65 kg ha-1 of Mg, at the 0-20 cm layer. NPK fertilization occurred in the corn planting, in line, below the seeds, on December 2017. The soil was collected in June 2017, after the harvest. Macronutrients, micronutrients and heavy metals contents were determinate in the diagnostic leaves of the plants as well as the shoots dry matter and corn yield. Soil organic matter (SOM), macro and micronutrients contents, pH and CEC were determinate in the soil. Nitrogen contents in the plants ranged from 28 to 30 g kg-1; while P ranged from 1.8 to 2 g kg-1 and K from 19 to 20 g kg-1. However no significative effects were identified for such elements in the diagnostic leaves. Furthermore, neither the corm yield nor the dry matter shoots were affected by the addition of WB to the soil. There was a small increase in the soil pH and CEC for the higher doses of WB, but also it were not significative. SOM did not increase with the WB applied doses, indicating that the increase of WB did not provide acceleration in SOM decomposition neither was mineralized. The results indicate that, after 14 months of soil application, WB aging in soil was not significant. In addition, the WB showed low neutralization power in soil, which is often with biochar application in tropical soils.
650 $asoil organic matter
650 $aEucalipto
650 $aFertilidade do Solo
650 $aMilho
650 $aOxisol
653 $aBy-product
700 1 $aCOSCIONE, A. R.
700 1 $aANDRADE, C. A. de
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